Corynebacterium glutamicum as platform for the production. Ellipses represent enzymes and transport systems present in c. Previous studies of the effect of gradients in scale. This updated second edition covers the molecular biology, genome engineering tools and comprehensive analysis techniques for corynebacterium glutamicum. Among strains of corynebacterium glutamicum, bacteria widely used for the industrial production of amino acids. Corynebacterium glutamicum an overview sciencedirect. A suitable reporter strain was transformed with a pyc gene library. Updates on industrial production of amino acids using. The grampositive prokaryote corynebacterium glutamicum has a long standing history in the industrial production of amino acids 14, 15. Glutamate production is accompanied by decreased 2oxoglutatate dehydrogenase activity. Corynebacterium glutamicum, an established microbial cell factory for the biotechnological production of amino acids, was recently genetically engineered for aerobic succinate production from glucose in minimal medium. Importance of nadph supply for improved lvaline formation in corynebacterium glutamicum.
Synthetic biology approaches to access renewable carbon source utilization in corynebacterium glutamicum nannan zhao, ling qian, guangjuan luo, suiping zheng applied microbiology and biotechnology 2018 102 22, 95179529. Corynebacterium glutamicum biology and biotechnology nami. Due to its importance as an amino acid producer, c. Indeed, several production processes for amino acids and related compounds had already been.
Cysteine synthase a cysk catalyzes the last reaction of l. Aromatic compound production by corynebacterium glutamicum. Corynebacterium glutamicum biology and biotechnology, edition. Allosteric regulation of phosphoenolpyruvate carboxylase pepc controls the metabolic flux distribution of anaplerotic pathways. Schematic presentation of the central carbon metabolism of c. Emerging corynebacterium glutamicum systems biology envis. Overexpression of mtr promotes resistance to oxidants, alkylating agents, bactericidal antibiotics, and heavy metals. Store search search title, isbn and author handbook of corynebacterium glutamicum by lothar eggeling, michael bott estimated delivery 312 business days format hardcover condition brand new one of the most important organisms in biotechnology, corynebacterium glutamicum is currently used to produce 2 million tons of amino acids per year for a rapidly expanding market. Biology and biotechnology microbiology monographs ed 2 english isbn. Corynebacterium glutamicum rite molecular microbiology and biotechnology group is seeking solutions in tackling the environmental problems by taking advantages of the useful bioconversion capability of microorganisms. Unfortunately, consistent with reports that mtr is an essential gene in m. Stephan noack, institute of bio and geosciences, ibg1. In particular lglutamate and llysine are produced at a million tonscale with this bacterium, but engineered strains for the production of other proteinogenic amino acids are also available 16, 17.
Amino acid production by corynebacterium glutamicum. Corynebacterium glutamicum juser forschungszentrum julich. The enzymes of the tricarboxylic acid tca and glyoxylate cycles of corynebacterium glutamicum and in particular their regulation have been intensively studied in the past years. Corynebacterium glutamicum biology and biotechnology. During growth in glucose minimal medium, iron limitation caused a shift from lactate to pyruvate as the major secreted organic acid complemented by lalanine and 2oxoglutarate. Mycolic acid ma plays important role in corynebacterium glutamicum, but the key enzymes in the biosynthetic pathway of ma in c. Indeed, several production processes for amino acids and related compounds had. Corynebacterium glutamicumstrain development has adopted each metabolic engineering tool immediately upon its conception and has contributed to developing these tools further. Largescale engineering of the corynebacterium glutamicum genome.
The response to iron limitation of the grampositive soil bacterium corynebacterium glutamicum was analyzed with respect to secreted metabolites, the transcriptome, and the proteome. Corynebacterium glutamicum rite molecular microbiology. Targeted topdown strategies for genome reduction are considered to have a high potential for providing robust basic strains for synthetic biology and industrial biotechnology. Overexpression of mycothiol disulfide reductase enhances. Journal of biotechnology 124 2006 7492 emerging corynebacterium glutamicum systems biology volker f. While originally identified in a screen for organisms secreting lglutamate, mutants of c. The actinobacterium corynebacterium glutamicum, an industrial workhorse article pdf available in journal of microbiology and biotechnology 265 february 2016 with 1,738 reads.
Evolutionary engineering of corynebacterium glutamicum. Leading corynebacteria experts provide a description of the current stateoftheart molecular biology toolbox available for corynebacterium research, including global analyses techniques such as comparative genomics, transcriptome, proteome and metabolome analysis as well as the most recent knowledge on corynebacterium promoter structures and vector systems. Despite being grampositive, the organization of the cell envelope resembles that of gramnegative bacteria. Cysteine synthase a overexpression in corynebacterium. Pyruvate carboxylase is an anaplerotic carbon dioxidefixing enzyme replenishing the tricarboxylic acid cycle with oxaloacetate during growth on sugars. Corynebacterium glutamicum is a wellstudied bacterium which naturally overproduces glutamate when induced by an elicitor. Based on the originally developed technology, represented by the rite bioprocess, we are working on the production of valuable chemicals from biomass, detoxification of. Corynebacterium glutamicum is the major host for biotechnological production of amino acids, the most important ones being the flavor enhancer lglutamate and the feed additive llysine. Aside from modern omicsbased approaches, the authors also focus on cell physiology, including cell division, central carbon metabolic pathways, and the respiratory chain. Corynebacterium glutamicum previously known as micrococcus glutamicus is a grampositive, rodshaped bacterium that is used industrially for largescale production of amino acids. During the half century following its discovery, the lglutamateproducing microorganism corynebacterium glutamicum has played a leading role in the amino acid fermentation industry. Based on the knowledge of the biochemical pathways microbial fermentation processes of food, feed and pharma amino acids have been developed. Industrial production of amino acid, a key sector of white biotechnology, is the classical field of application of corynebacterium glutamicum.
Recently, we created a library of 26 genomereduced strains of corynebacterium glutamicum carrying broad deletions in single gene clusters and showing wildtypelike biological fitness. Glycerol as a substrate for aerobic succinate production. Corynebacterium glutamicum was discovered in japan in 1956 as a natural glutamate producer. Pdf the actinobacterium corynebacterium glutamicum, an. Wendisch a michael bott a jorn kalinowski c marco oldiges b wolfgang wiechert d. Tca cycle and glyoxylate shunt of corynebacterium glutamicum. Download corynebacterium glutamicum biology and biotechnology. In this study, we applied the industrial workhorse corynebacterium glutamicum, which has a long tradition in biotechnological production of amino acids but is also exploited for the biosynthesis of organic acids, alcohols and specialty chemicals liebl, 2005. Your print orders will be fulfilled, even in these challenging times. In this work, the corresponding strains were transformed with plasmid pvwex1. Pyruvate carboxylase variants enabling improved lysine. Applied microbiology and biotechnology 2018, 102 9, 39153937. In this study, cysk was overexpressed in corynebacterium glutamicum iwj001, an l.
They are bacilli rodshaped, and in some phases of life they are, more particularly, clubshaped, which inspired the genus name coryneform means clubshaped they are widely distributed in nature in the microbiota of animals including the human. The iron deficiency response of corynebacterium glutamicum. The discovery of corynebacterium glutamicum during a search initiative for glutamate. Metabolic engineering in corynebacterium glutamicum. Harnessing novel chromosomal integration loci to utilize.
Based on rational protein design, six pepc mutants were designed, and all of them showed. Specific engineering strategies with application of industrial biotechnology will be discussed in more detail in other chapters of this book. Walter keller, in progress in molecular biology and translational science, 2011. In this study, we applied a lysine biosensor to identify pyruvate carboxylase variants in corynebacterium glutamicum that enable improved llysine production from glucose. Building and testing a novel platform host for synthetic biology and industrial biotechnology. Corynebacterium glutamicum is most widely known for its role in the industrial. Its microbial factory qualities, such as its physiological plasticity and robust catalytic functionalities, have since facilitated the development of efficient production processes.
Metabolic engineering of escherichia coli, corynebacterium glutamicum, pseudomonas, bacillus and yeast strains for access to alternative. The flexible feedstock concept in industrial biotechnology. Packed with practical information and stateoftheart science, this concise volume is an essential handbook for everyone working with corynebacterium and related organisms in academia, biotechnology companies, and the pharmaceutical industry, it is a recommended volume for all microbiology libraries. Production strains of corynebacterium glutamicum, which has been used safely for more than 50 years in food biotechnology, and escherichia coli are constantly improved using metabolic engineering approaches.
Deregulation of feedback inhibition of phosphoenolpyruvate. Metabolic engineering of corynebacterium glutamicum for fermentative production of chemicals in biorefinery. Cell division mechanism of corynebacterium glutamicum, publisher. Its microbial factory qualities, such as its physiological plasticity and robust catalytic functionalities, have since facilitated the development of efficient production processes for amino acids, nucleotides and vitamins. Emerging corynebacterium glutamicum systems biology. A new era in corynebacterium glutamicum biotechnology. Slayers also occur on the biotechnologically relevant corynebacterium glutamicum. Corynebacterium glutamicum wikipedia, a enciclopedia libre. Production of 2ketoisocaproate with corynebacterium.
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